›› 2011, Vol. 23 ›› Issue (6): 452-455.doi: 10.3969/j.issn.1004-616x.2011.06.012

• 核辐射安全专家论坛 • Previous Articles     Next Articles

A feasibility study on rapid radiation damage estimation using peripheral blood reticulocytes and T cell subsets

WANG Lei1，2，WANG Zhi-dong，HU Hai-liang，ZHANG Xue-qing，CHEN Mao-sheng，XUE Shao-li，CHEN Ying   

  1. 1. Department of Medical Biotechnology, Anhui Medical University, Hefei 230032, Anhui; 2. Insititute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
  • Received:2011-09-19 Revised:2011-10-19 Online:2011-11-30 Published:2011-11-30
  • Contact: XUE Shao-li

PDF (PC)

959

Abstract: OBJECTIVE: To identify the changes of the percentage of reticulocytes and Th-to-Ts ratios (rTh/Ts) from peripheral blood in mice following total body irradiation(TBI) in order to confirm the feasibility of bio-dosimeter about radiation damage. METHODS：Seven to eight weeks-old C57BL/6 mice were treated with TBI at doses ranging from 1 Gy to 7Gy. Peripheral blood was collected for flow cytometric analysis，percentage of reticulocytes and rTh/Ts were acquired. RESULTS：Reduced reticulocyte percentages were observed with the doses in the range of 0～7 Gy post-irradiation，minimal reticulocyte percentages were observed at 3 d，and the recovery of reticulocytes was increasingly delayed with higher radiation doses. RTh/Ts increased with doses in the range of 0～7 Gy within 72 h post-irradiation. Percentage of reticulocytes declined with the doses in the range of 1～3 Gy at 24，48 and 72 h post-irradiation. rTh/Ts increased with doses in the range of 1～7 Gy at 6，24 and 72 h after TBI. The dose-response relationships of both reticulocyte percentage and rTh/Ts fitted to the lineal model. CONCLUSION：Percentage of reticulocytes and rTh/Ts ratios analyzed with flow cytometry，could be an early，rapid and high-throughput radiation bio-dosimeter.

Key words: radiation, bio-dosimeter, flow cytometry, reticulocyte, T cells subset